One of the biggest challenges faced by the regenerative medicine field today is the ability to efficiently and scalably generate therapeutic cells. This session explores cutting-edge transcription factor-based approaches for rapid and efficient differentiation of stem cells into therapeutic cell types. We will examine how synthetic biology techniques are revolutionizing cell therapy by enabling faster production of clinically relevant cells. Speakers will discuss recent advances, challenges, and future directions in harnessing transcription factors for directed differentiation, with a focus on applications in regenerative medicine and drug discovery.